Strategy for results
| 1 | Cloning of gRNA into plasmid | ||
| 2 | Validation by sequencing | ||
| 3 | Midiprep | ||
| 4 | Tissue culture | ||
| 5 | Transfection and selection | ||
| 6 | DNA isolation | ||
| 7 | Sonication | ||
| 8 | Treatment with enzymes HpaII | ||
| 9 | qPCR | ||
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